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How to Grow Mushrooms From a Spore Syringe

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Spore syringes can be used to store spores of any mushroom strain. The spores in a syringe are millions and float in a sterile solution. It is very simple to use a spore-syringe.
Inoculating the substrate is done with a mushroom spore needle syringe. A spore syringe can be used to cultivate any mushroom strain, whether it is magic mushrooms, oyster mushrooms, medicinal strains or medicinal.
How to prepare the Spore Syringe

First, ensure that you have a clean environment. Before you prepare the mushroom spore needle syringe, wash your hands and use sterilized gloves.

Spore syringes are usually ready to use. It is best to store it in the fridge for 12 hours before bringing it out at room temperature.

Usually, a spore syringe package includes:

1x Sterile needle
1x Special mushroom spore solution, stored in a 20ml syringe
4x Alco preps

Liquid spore syringe set

A Luer lock is the cap on a sealed syringe. This is how it works.

Keep the cap on the spore syringe.
Keep it in place with the Luer lock pointed up.
Next, turn the syringe counterclockwise using your hand to unscrew its lock.
Remove the needle from its sterile packaging (leave the protective cap off the needle) and attach it to the mushroomspore syringe.
For a few seconds, shake the spore-syringe.

Now it is ready to inoculate (sterile) substrate.
How to Innoculate Substrate With Your Spores Syringe

Different substrates are required for different strains of edible, psilocybe, or medicinal mushrooms.

To colonize 1 liter or 1.05 quarts of substrate, you will need 3 milliliters worth of the spore mixture. A syringe contains 20 ml mushroom spore solutions. This is sufficient to inoculate 6-7 liters of substrate. All you have to do is drop a few milliliters of the mushroom spore solution in each corner of your substrate, and it will set everything.

The mushroom spores dropped on the area where mycelium has grown over the next few days will show white spots. When the substrate becomes white, then mycelium is fully established.

By cutting the bag, you can choose the spot where the mushrooms will emerge from. This will initiate the flowering process.
How do you store your mushroom-spore syringe?

The spore syringe can be stored in a refrigerator between 2 and 8 degrees C (Celsius), or 35 to 46 degrees F (Fahrenheit). After using the spore syringe, make sure to attach the Luer lock again. The Syringe is good for 12 months.


Use the ape spores Syringe only for liquid culture. If you wish to make liquid culture using the mushroom spores from the syringe, either use the spore vial (or print it out)
How long does a Liquid Spore Culture last?

The spores of mushrooms can survive for many years. If you plan to keep them for the long-term, make sure you cool them off. Before you put them in the refrigerator, make sure they are sealed to prevent moisture. Their shelf life will be extended by sealing them against heat and air. Prints and spore vials can last for more than a decade.

The spore syringes won’t last as long before the water starts to grow bacteria. A good timeline is between 8 and 12 months. If your spore fails to grow in the time expected, you can rehydrate in sterilized water for approximately 24 hours. For spores older than six months, you can soak them in sterile water for 24 hours to ensure germination. Liquid culture syringes should be used within three months after purchase. Keep them in the refrigerator.